Correct Peptide Reconstitution

Peptides are delicate biomolecules that require precise handling and storage to maintain their integrity and effectiveness. Our peptides are delivered as lyophilized (freeze-dried) powders, which offer stability against short-term temperature fluctuations during transit. Even at room temperature, these powdered peptides remain stable for several weeks. However, to maximize their shelf life and ensure consistent results, proper storage and reconstitution methods must be followed.

Storage and Handling Best Practices

Before Reconstitution

Store lyophilized peptides in a dry, cool place, preferably at −20°C to −80°C for long-term preservation. This prevents degradation and contamination.

After Reconstitution

Once peptides are reconstituted, they become more susceptible to degradation. Store solutions at 4°C for short-term use (a few days) or freeze aliquots at −20°C for longer periods.

How to Reconstitute Peptides Properly

1. Choose the Right Solvent

The solvent choice depends on the peptide's solubility:

• Bacteriostatic water — Suitable for most peptides, but may not dissolve highly hydrophobic peptides completely.
• Acetic acid (0.1%) or dilute HCl — Used sparingly for highly hydrophobic peptides.
• Other buffers — Used when specific pH conditions are required.

2. Gently Add the Solvent

Add solvent slowly to the side of the vial (dropwise) to avoid foaming. Mix by gently swirling or tilting — do not vortex or shake aggressively.

3. Check for Clarity

Ensure the solution is clear and free of undissolved particles. If needed, brief sonication can help dissolve stubborn peptides.

Common Mistakes to Avoid

• Using tap or non-sterile water — introduces contaminants.
• Shaking the vial aggressively — physically degrades the peptide.
• Letting the reconstituted solution sit at room temperature for extended periods.
• Not aliquoting solutions for storage, leading to repeated freeze–thaw cycles.